Shannon Eagle

Shannon Eagle, MSc. (Laboratory Manager)

I completed my B.Sc. in Molecular Biology and Genetics and my M.Sc. in Evolutionary Biology both at the University of Guelph.

As an undergraduate student, I worked in the Hajibabaei lab. There I performed subsampling, DNA extractions, PCRs, gel electrophoresis, DNA sequencing, and DNA sequence editing. In addition, I participated in the natural health products project.

I went on to do an undergraduate research project with Dr. Teresa Crease. During my undergraduate project, I learned how to do qPCR and molecular cloning. My undergraduate project used qPCR to determine whether there was a relationship between the number of rDNA copies and the number of DNA transposons, Pokey, inserted within rDNA. In addition, I used a modified AFLP method, transposon display, to look at the number and distribution of Pokey within Daphnia species.

I remained in the Crease lab for my M.Sc and expanded upon the research I conducted as an undergraduate. For my M.Sc. research project, I used qPCR to determine the copy number of each member of the Pokey family across the Daphnia pulex species complex. In addition, I used a modified yeast 2 hybrid assay to determine the excision rate of various Pokey transposons.

Following the completion of my M.Sc., I worked for a year as a Quality Systems Coordinator with ALS Environmental. At ALS, an ISO/IEC 17025 accredited laboratory, I maintained the quality system.

As Laboratory Manager in the Hajibabaei laboratory, I plan and perform environmental sample processing for DNA metabaroding.

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